Bacillus Megaterium

An efficient alternative to E. coli: Stable protein expression with high yield - suited not only for industrial scale.

MoBiTec offers this expression system as an easy-to-handle kit with E. coli/B. megaterium

shuttle vectors and - to be ordered separately - B. megateriumprotoplasts ready for transformation.

• Stable, high yield protein production
• Suited for small to industrial-scale protein production
• Tightly regulated and efficiently inducible xylA operon (up to 350-fold)
• No endotoxins are found in the cell wall
• No indication of proteolytic instability even up to 5 h after induction, since alkalineproteases such as e.g. in B. subtilisare not produced
• Extended polylinker downstream of promoter allows versatile cloning
• Additional Bsr GI site enables cloning without modifying the N-terminus
• Plasmids available for either intracellular or extracellular production
• Easy purification and detection of either 6xHis, Strep-tagged orStrep-/6xHis-double-tagged target proteins
• Removable purification tags due to TEV and Factor Xa sites
• Compatible with all Bacillus subtilis vectors

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High yield protein expression with the T7 RNA polymerase expression system

• Stable, high yield protein production
• Suited for small to industrial-scale protein production
• Tightly regulated and efficiently inducible xylA operon / T7 RNA polymerase
• No endotoxins are found in the cell wall
• No indication of proteolytic instability even up to 5 h after induction, since alkaline
• proteases such as e.g. inB. subtilis are not produced
• Easy transformation by use of pretransformed B. megaterium protoplasts
• Control vector with GFP-sequence included in the kit