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taq polimerasi - wikipedia
La Taq polimerasi (DNA polimerasi termostabile) è una polimerasi, proveniente dall'organismo termofilo Thermus aquaticus.L'enzima è costituito da una singola catena polipeptidica, con massa molecolare di 94 kDa. Presenta un'attività principale DNA-polimerasi DNA-dipendente in direzione 5'→3', per la quale è richiesta una temperatura ottimale di 75-80 °C e la presenza di ioni Mg 2+ (ad es.
taq dna polymerases | nebFlexibility. Taq DNA Polymerase is the industry standard for routine PCR.Taq with Standard Taq Buffer is available in economical extra-large pack sizes.NEB provides high quality recombinant Taq at an exceptional value.Taq is available with different formats to accommodate a variety of PCR applications. For example, Taq with standard Taq Buffer is designed to support existing PCR platforms ...
taq polymerase - wikipediaTaq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol.It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA.
Altri risultati:
hotstartaq dna polymerase - qiagenHotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. HotStarTaq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer, which minimizes nonspecific amplification products, primer ...
taq polymerase - an overview | sciencedirect topicsRichard D. Abramson, in PCR Strategies, 1995 Taq DNA Polymerase. Taq DNA polymerase is an 832-amino acid protein with an inferred molecular weight of 93,920 and a specific activity of 292,000 units/ mg; optimal polymerization activity is achieved at 75–80 ° C, with half-maximal activity at 60–70 ° C (Lawyer et al., 1993; see also Table 1).It's thermostability as measured as a half-life ...
taq polymerase: what is it & what does it do? - excedrNo Taq polymerase. No PCR. PCR amplification works on the principle of temperature variation—heating and cooling reactions—which makes Taq polymerase a highly advantageous enzyme. The major reason behind this is that Taq polymerase can work at high temperatures with high efficiency and amplification capacity, which other bodily enzymes cannot.
taq dna polimerasi: la rivoluzione della biologia molecolareInfatti la Taq polimerasi è un enzima termostabile che non si degrada alle alte temperature, a differenza della DNA polimerasi. Proprio per la sua capacità di tollerare alte temperature, la Taq polimerasi è l’enzima più utilizzato per sintetizzare il DNA o il gene di interesse in vitro mediante la tecnica PCR.