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taq polimerasi - wikipedia
La Taq polimerasi (DNA polimerasi termostabile) è una polimerasi, proveniente dall'organismo termofilo Thermus aquaticus.L'enzima è costituito da una singola catena polipeptidica, con massa molecolare di 94 kDa. Presenta un'attività principale DNA-polimerasi DNA-dipendente in direzione 5'→3', per la quale è richiesta una temperatura ottimale di 75-80 °C e la presenza di ioni Mg 2+ (ad es.
taq polymerase - wikipediaTaq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol.It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA.
taq dna polymerases | nebFlexibility. Taq DNA Polymerase is the industry standard for routine PCR.Taq with Standard Taq Buffer is available in economical extra-large pack sizes.NEB provides high quality recombinant Taq at an exceptional value.Taq is available with different formats to accommodate a variety of PCR applications. For example, Taq with standard Taq Buffer is designed to support existing PCR platforms ...
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il ruolo della taq polimerasi nella pcrTaq polimerasi, la prima DNA polimerasi termostabile per La PCR è stata scoperta nel 1966. La PCR ha trasformato l'amplificazione del DNA, rendendo il processo rapido ed efficiente. Ciò rivoluzionerebbe la clonazione, il test del DNA, la medicina legale e il design della medicina.
taq buffer (10x), with kcl - thermo fisher scientificThermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.10X Taq Buffer with KCl (B38) includes: 100 mM Tris-HCl (pH 8.8 at 25C) 500 mM KCl 0.8% (v/v) Nonidet P40Related
mytaq™ dna polymerase | bioline | meridian bioscienceMyTaq DNA Polymerase is a high performance polymerase that exhibits more robust amplification than other commonly used polymerases, delivering very high yield over a wide range of PCR templates and making it the ideal choice for most PCR assays.
taqi (10 u/µl) - thermo fisher scientificTaqI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain, such as GM2163. Incubation at 37°C results in 10% activity. We recommend using TaqI, HC at 37°C. For methylation sensitivity, refer to product specifications.